3.3 In vitro cell contact toxicity of optimum SilMA
After the above procedures, the concentration of 20% was selected. The cell contact toxicity test was operated to evaluate the cytocompatibility of optimum SilMA. The fusiform adherent cells were obviously observed 24 hours after cell seeding, as shown in Fig. 6a-d. After 48 hours of co-culture with the samples, the morphology of cells began to appear difference. The cells around the PCL and SilMA grew well and showed a long shuttle clustered growth [Fig. 6(f, g)]. Compared with blank control (Fig. 6e), no matter in morphology or density of cells, there was no obvious difference. However, there were almost no adherent cells in the positive control group (Fig. 6h). This result was more obvious after Giemsa staining, which can dye the nucleus of the cells purple or blue purple, while the cytoplasm became pink (Fig. 6i-k). In Fig. 6l, the remaining cells showed the round or irregular shape, vacuoles were observed in the cytoplasm, and the nuclei were pyknotic.