Figure 3. Overexpression of Lf in astrocytes inhibits APP phosphorylation in APP/PS1 mice. (A) The expression of p-APP (Thr668) was downregulated, while the expressions of APP, BACE1, ADAM10, and PS1 remained similar in APP/PS1/Lf mice compared to APP/PS1 mice. n = 6; Two-way ANOVA followed by the Bonferroni’s post hoc test. (B) Double immunostaining showing the reductions of p-APP expression in the Aβ plaques and neurons in APP/PS1/Lf mouse cortex. White arrows indicate the neurons. n = 5. (C) Overexpression of Lf in astrocytes reduced the expression of sAPPβ, but did not affect the expression of sAPPα in APP/PS1 mice. n = 6; Two-way ANOVA followed by the Bonferroni’s post hoc test. (D) Overexpression of Lf in astrocytes promoted the expression of APOE in WT mice, and reversed the downregulation of LRP1 and IDE in APP/PS1 mice. n = 6; Two-way ANOVA followed by the Bonferroni’s post hoc test. *P < 0.05, **P < 0.01.