Figure 3. Overexpression of Lf in astrocytes inhibits APP
phosphorylation in APP/PS1 mice. (A) The expression of p-APP (Thr668)
was downregulated, while the expressions of APP, BACE1, ADAM10, and PS1
remained similar in APP/PS1/Lf mice compared to APP/PS1 mice. n = 6;
Two-way ANOVA followed by the Bonferroni’s post hoc test. (B) Double
immunostaining showing the reductions of p-APP expression in the Aβ
plaques and neurons in APP/PS1/Lf mouse cortex. White arrows indicate
the neurons. n = 5. (C) Overexpression of Lf in astrocytes reduced the
expression of sAPPβ, but did not affect the expression of sAPPα in
APP/PS1 mice. n = 6; Two-way ANOVA followed by the Bonferroni’s post hoc
test. (D) Overexpression of Lf in astrocytes promoted the expression of
APOE in WT mice, and reversed the downregulation of LRP1 and IDE in
APP/PS1 mice. n = 6; Two-way ANOVA followed by the Bonferroni’s post hoc
test. *P < 0.05, **P < 0.01.