Figure 2. Overexpression of Lf in astrocytes promotes Lf secretion into neurons. (A) The expression of Lf was unchanged between the WT mouse brains and APP/PS1 mouse brains at the age of 10-months old. n = 6; Student’s t -test. (B) The contents of total Lf (mouse Lf and human Lf) and human Lf were increased in APP/PS1/Lf mouse brains. n = 6; Student’s t -test. (C) The contents of cellular and secreted Lf were increased in the mouse primary astrocytes from Astro-Lf mice. n = 5; Student’s t -test. (D) The human Lf was overexpressed in the astrocytes from APP/PS1/Lf mouse brains. n = 5. (E) Human Lf was observed in the NeuN-positive cells in APP/PS1/Lf mouse brains. n = 5. **P < 0.01.