2.7. Frequency analysis of mutations in SARS-CoV-2 spike
glycoprotein corresponding to the mutated mAb epitope residues
Data from the GISAID hCoV-19 spike glycoprotein mutation surveillance
dashboard was obtained for all spike protein variations in SARS-CoV-2. A
total of 81,79,987 SARS-CoV-2 spike sequences (updated on February 19,
2022, by Raphael Tze Chuen Lee, GISAID) were compared to the reference
sequence EPI_ISL_402124 for the annotation of individual mutations
(mutation data obtained from GISAID). Among the spike glycoprotein
mutations, the residues that are mAb binding sites as well as mutated in
VOCs were analysed for naturally occurring mutations. The occurrence
reported for each such mutation was divided by the total number of
sequences (n=8179987) and converted into percentage. The mutations
occurring at these particular residues with a frequency greater than
0.01% were selected for plotting. A stacked plot was generated with
series in ascending order of frequency percentage values. Y-axis
represents the frequency in logarithmic scale while X-axis represents
the mutated mAb binding sites in the spike glycoprotein.