2.6. Frequency analysis of the neutralizing mAb epitope residues
Frequency of 74 experimentally validated mAb epitope residues occurring
in SARS-CoV-2 spike protein was computed using python script. Briefly,
the excel file with the details of epitope residues was processed using
the pandas library [15]. Frequency of independent epitope residues
was calculated and normalized by the total number of antibodies (n=74)
and converted into percentage. The percentage frequency for those
epitope residues that have been observed to be mutated in the SARS-CoV-2
spike variants of concern was plotted as a bar plot using the pyplot
module in the python graphical plotting library Matplotlib [14]. To
generate the colorbar for frequency, percentage frequency values were
normalized by the maximum value among the computed frequency and ‘jet’
colormap was used to plot the colorbar using Scalar Mappable class.