Golgi-Cox Staining
After the mice have been anesthetized with 10% chloral hydrate (400
mg/kg) (Aladdin, China), brains were removed quickly from the skull,
rinsed with 0.1M PBS. Staining of brain slices was performed according
to the manufacturer’s instructions using the FD Rapid Golgi Stain kit
(FD Neuro Technologies, USA). Brains were cryosectioned coronally into
100 µm sections containing the hippocampal area with a cryostat
microtome (Leica, Germany). The slices were mounted on gelatin-coated
microscope slides, stained, dehydrated, and covered with Vecta Mount
Mounting Medium (Vector, USA).