Sholl Analysis
Sholl Analysis is a classical method to analyze neuronal complexity by
drawing a series of concentric circles with the soma as the center. This
is done by obtaining the number of intersections of dendrites that have
distances at 10mm intervals from the soma. The number of intersections
is used to reflect the complexity of the cell. We used Imaris software
(version 7.7.2) for dendritic complexity sholl analysis. The steps for
dendritic complexity sholl analysis are as follows: color
inversion–select the soma as a center–select the dendritic
end–perform semi-automatic dendritic analysis. Dendrite branching
should be flipped to 3D to match the selection in order to distinguish
real dendrite branches. When the drawing is finished, select specific
values in the statistics tab - filament No. sholl intersections (Pillaiet al. , 2012).