Fig.3 gRNA precursor processing ability of Cas13b/d. (A) The scheme showing Cas13 processes the concatenated gRNA precursors into mature gRNAs. u,m,d are gRNAs at up, mid and downstream locations respectively. The “+” between gRNA symbols indicates that it is a mixture of corresponding gRNA monomers, otherwise it is the precursor of corresponding gRNA. (B-E) Effects of gRNAs form on Cas13b. (F-I) Effects of gRNAs form on Cas13d. The results are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.