Loss of endothelial cell Gch1 and BH4 lead to progressive
hypertension during pregnancy.
Consistent with our previous reports
(Chuaiphichai et al., 2021), we observed a
small but significant increase in basal systolic blood pressure
(~5-7 mmHg) in Gch1fl/fl Tie2cre
mice compared with wild-type littermates (WT: 103 ± 2 mmHg, vsGch1fl/fl Tie2cre mice: 110 ± 1 mmHg,P <0.05, n=6; Figure 1A). In wild type mice, systolic
blood pressure was unchanged during pregnancy (baseline WT: 103 ± 2
mmHg, vs pregnant WT: 103 ± 3 mmHg, n=6; Figure 1A). In contrast, loss
of endothelial cell Gch1 resulted in a significantly marked
increase (~24 mmHg) in systolic blood pressure in later
pregnancy in Gch1fl/fl Tie2cre (Figure 1A).