Figure Legend
Figure 1. Bone marrow core biopsy specimen(A-F). Sheets of immature
blasts on H&E stains (A, ×200; B, ×400), with a subset positive for
CD34 (C, ×200), TdT (D, ×200), CD10 (E, ×200), CD20(F, ×200).
Bone marrow and peripheral blood smears (G-J). Bone marrow aspirations
showed sheets of small- to intermediate-sized blasts with high nucleus
to cytoplasmic ratio and dispersed nuclear chromatin on March 30,2021(G)
and April 16,2021(H). Peripheral blood smears showed atypical lymphoid
cells with immature-appearing chromatin on March 30,2021(I) and April
16,2021(J).
Figure 2. Flow cytometry immunophenotyping of the blasts in peripheral
blood and bone marrow. No blasts were presented in peripheral blood on
March 23(A). Peripheral blood showed CD45 dim cells with TdT, CD123,
CD19 and bright CD10 expression on April 16 (B-D). In bone marrow, a
characteristic aberrant lymphoblast phenotype with CD34, CD19, CD10,
CD22 and CD20(partial) positive on March 30(E-H).
Figure S1. Suppresion T subtypes
assays by flow cytometry. On March 30 (A-C) and April 16 (D-F), flow
cytometry immunophenotyping in peripheral blood showed a higher
proportion of CD3+CD4+CD25+Foxp3+ Treg(A,D), CD3+TCRαβ+CD4-CD8- DNT(B,E)
and CD3+CD4+CD185+CD279+ TFH(C,F).