Human rhabdomyosarcoma cells |
Trypsinization/three times with
ice-cold PBS |
Liquid nitrogen |
10% ice-cold TCA |
Nuclear magnetic
resonance (NMR) |
[50] |
Fibroblasts |
No |
Methanol: water 80:20 (80% methanol) at −75
°C |
Methanol: water 80:20 (80% methanol) at −75 °C |
LC-MS/MS |
[46] |
CHO |
No |
60% methanol at −40 °C |
100% methanol |
Gas
chromatography/time of flight-mass spectrometry (GC-TOF-MS) |
[24] |
MCF-7 and MDA-MB-231 cells |
Twice with ice-cold
phosphate-buffered saline (PBS, pH 7.4) |
High Performance Liquid
Chromatography (HPLC) grade methanol |
A mixture of methanol, chloroform
and water in the volume ratio of 4:4:2.85 |
NMR |
[47] |
HCT116 |
Once with PBS |
Liquid nitrogen |
Cold 80% methanol |
LC-MS |
[51] |
HCT116 |
Washed (20 s) with washing buffer (140 mM NaCl, 5 mM
HEPES, pH 7.4, 37 °C) |
Ice-cold 50% MeOH solution |
1:1:1, v/v/v MeOH:
CHCl3: H2O |
GC-MS |
[20] |
Breast cancer cell lines |
Washed twice with a washing solution
(75 mM ammonium carbonate, adjusted to pH 7.4 with Acetic Acid) |
Liquid
nitrogen |
70% (v: v) ethanol: water at 75 °C |
Flow injection analysis
time-of-flight mass spectrometry |
[52] |
CHO |
No |
60% methanol supplemented with 0.85% (w/v) AMBIC
(pH 7.4) at −40°C |
Methanol + water |
GC-MS |
[26] |
SW480 |
No |
Liquid nitrogen |
80% methanol, 20% water
(MeOH/H2O) |
GC-MS |
[18] |
Clonal β-cell line INS-1 |
37 °C deionized water (∼2 s) |
liquid nitrogen |
9:1 methanol: chloroform |
HPLC-MS |
[49] |
Recombinant CHO cell line Super-CHO |
Washed twice with PBS at
room temperature |
Cold 0.9% (w/v) NaCl |
Cold extraction using 50%
acetonitrile |
HPLC |
[48] |