2.8 Data statistical analysis
All data in this study were expressed as mean ± standard deviation (SD). Data analysis was performed by GraphPad Prism 8.0. The heatmap was generated using the pheatmap package (R version 4.0.1). The pathway enrichment was performed using the MetaboAnalyst (https://www.metaboanalyst.ca/). Significant analysis was performed by GraphPad Prism 8.0, with p < 0.05, p < 0.01 and p < 0.001 indicated that there were significant (*), highly significant (**) and extremely significant (***) difference between the groups, respectively.