Cell type Rinse Quenching Extraction Analytical technique Ref.
Human rhabdomyosarcoma cells Trypsinization/three times with ice-cold PBS Liquid nitrogen 10% ice-cold TCA Nuclear magnetic resonance (NMR) [50]
Fibroblasts No Methanol: water 80:20 (80% methanol) at −75 °C Methanol: water 80:20 (80% methanol) at −75 °C LC-MS/MS [46]
CHO No 60% methanol at −40 °C 100% methanol Gas chromatography/time of flight-mass spectrometry (GC-TOF-MS) [24]
MCF-7 and MDA-MB-231 cells Twice with ice-cold phosphate-buffered saline (PBS, pH 7.4) High Performance Liquid Chromatography (HPLC) grade methanol A mixture of methanol, chloroform and water in the volume ratio of 4:4:2.85 NMR [47]
HCT116 Once with PBS Liquid nitrogen Cold 80% methanol LC-MS [51]
HCT116 Washed (20 s) with washing buffer (140 mM NaCl, 5 mM HEPES, pH 7.4, 37 °C) Ice-cold 50% MeOH solution 1:1:1, v/v/v MeOH: CHCl3: H2O GC-MS [20]
Breast cancer cell lines Washed twice with a washing solution (75 mM ammonium carbonate, adjusted to pH 7.4 with Acetic Acid) Liquid nitrogen 70% (v: v) ethanol: water at 75 °C Flow injection analysis time-of-flight mass spectrometry [52]
CHO No 60% methanol supplemented with 0.85% (w/v) AMBIC (pH 7.4) at −40°C Methanol + water GC-MS [26]
SW480 No Liquid nitrogen 80% methanol, 20% water (MeOH/H2O) GC-MS [18]
Clonal β-cell line INS-1 37 °C deionized water (∼2 s) liquid nitrogen 9:1 methanol: chloroform HPLC-MS [49]
Recombinant CHO cell line Super-CHO Washed twice with PBS at room temperature Cold 0.9% (w/v) NaCl Cold extraction using 50% acetonitrile HPLC [48]