FIGURE 1. Sanger sequencing chromatograms of C14orf166cDNAs fused with GAL4-AD isolated from the yeast cells and amino acid
sequences of the proteins encoded from these cDNAs. NTR ,
non-translated region of C14orf166 gene.
Therefore, in this work, we focused on the interaction patterns of human
C14orf166 with the viral PA protein. Representative structures of the
proteins with different sizes encoded from the cDNAs are shown in Figure
2A. It was determined that some of the cDNAs encode the C14orf166
carboxy-terminal region, which consists of only 69 amino acid residues
fused with GAL4-AD (AD-C69). Some of the cDNAs encode the C14orf166
protein fused with GAL4-AD, which is composed of 209 amino acids lacking
35 residues (AD-C209), while some encode the protein lacking only three
amino acids at the amino-terminal end (AD-C14orf166/241). Sequencing
results showed that some of the cDNAs encode the full-size C14orf166
proteins having extra regions at the amino-terminal end consisting of 4
or more irrelevant amino acid residues corresponding to the
non-translated region (NTR) of the gene before the initial methionine
residue in the protein (AD-C244+4). Although in different sizes, the
C14orf166 proteins fusing with GAL4-AD caused an increase in reporter
β-galactosidase activity by interacting with bait PA protein in the
yeast cells (Figure 2B).