FIGURE 6. 3D models of influenza virus PA and AD-C69 proteins, and selected two docking patterns. A/C : several AD-C69 proteins clustered on the PA model, B/D : single AD-C69 protein-PA model.
As seen in Figure 2, some of the C14orf166 proteins interacting with the bait PA protein in the yeast cells consisted of 209 amino acid residues fused with GAL4-AD (AD-C209) while some were full-size (AD-C244+4) and carried additional amino acid residues corresponding to the NTR region of the gene. The interaction models of these two proteins of different sizes with the viral PA protein were predicted by in silico tools. For these proteins, 3D models were generated with I-TASSER, prominent 3D models were matched with the reference PA model using ClusPro, and interaction models of AD-C209:PA and AD-C244+4:PA are given in Figure 7.