FIGURE 1. Sanger sequencing chromatograms of C14orf166cDNAs fused with GAL4-AD isolated from the yeast cells and amino acid sequences of the proteins encoded from these cDNAs. NTR , non-translated region of C14orf166 gene.
Therefore, in this work, we focused on the interaction patterns of human C14orf166 with the viral PA protein. Representative structures of the proteins with different sizes encoded from the cDNAs are shown in Figure 2A. It was determined that some of the cDNAs encode the C14orf166 carboxy-terminal region, which consists of only 69 amino acid residues fused with GAL4-AD (AD-C69). Some of the cDNAs encode the C14orf166 protein fused with GAL4-AD, which is composed of 209 amino acids lacking 35 residues (AD-C209), while some encode the protein lacking only three amino acids at the amino-terminal end (AD-C14orf166/241). Sequencing results showed that some of the cDNAs encode the full-size C14orf166 proteins having extra regions at the amino-terminal end consisting of 4 or more irrelevant amino acid residues corresponding to the non-translated region (NTR) of the gene before the initial methionine residue in the protein (AD-C244+4). Although in different sizes, the C14orf166 proteins fusing with GAL4-AD caused an increase in reporter β-galactosidase activity by interacting with bait PA protein in the yeast cells (Figure 2B).