FIGURE 3. De novo 3D models of the carboxyl-terminal end
of C14orf166 consisting of 69 amino acid residues (C69) determined with
the I-TASSER algorithm. The carboxyl-terminal and amino-terminal ends of
the peptides are shown in red and blue, respectively. C-scores of the
peptides are C69-1: -3.05, C69-2: -2.78, C69-3: -3.24.
The C14orf166 3D, a reference model predicted by AlphaFold, also has a
longer alpha helix structure at the c-terminal end (Figure 4A). The
c-terminal end of this model has a similar folding pattern to the model
given in Figure 3, C69-1. The homology model of influenza A DkPen virus
PA protein generated with I-TASSER was used as a reference model for
PA:C14orf166 docking analyses (Figure 4B).