2.15. Sirius Red staining
Paraffin sections were de-waxed and hydrated. The nuclei were stained
with Weigert’s hematoxylin for 8 minutes, and then washed in running
water for 10 minutes. The slices were stained in picro sirius red for
one hour and washed with acidified water twice. Physically remove most
of the water from the slice by vigorous. The slices were dehydrated with
100% ethanol for three times, and finally transparented in xylene, and
sealed with neutral gum. Images were taken using ordinary forward
microscopy (Olympus, BX41).