2.2. Cell Viability Assay using CCK8
Ox-LDL (YB-002; Yiyuan Biotechnologies, Guangzhou, Guangdong, China) was dissolved in SMCGS-free SMCM at different concentrations. VSMC-derived foam cells were treated with 0, 1, 10, and 100 μg/mL ox-LDL for 24 h. Next, 10 µL of CCK8 (Dojindo, Kumamoto, Japan) solution was added to each well and incubated for 2 h. The absorbance of the samples was measured at 450 nm using a Tecan Infinite M200 Microplate Reader (LabX, Austria). All experiments were performed in triplicate and repeated thrice.