2.15. Sirius Red staining
Paraffin sections were de-waxed and hydrated. The nuclei were stained with Weigert’s hematoxylin for 8 minutes, and then washed in running water for 10 minutes. The slices were stained in picro sirius red for one hour and washed with acidified water twice. Physically remove most of the water from the slice by vigorous. The slices were dehydrated with 100% ethanol for three times, and finally transparented in xylene, and sealed with neutral gum. Images were taken using ordinary forward microscopy (Olympus, BX41).