Figure C 2: GC-MS Chromatogram for Geranyl benzoate
GC-MS analysis including chromatograms of all alcoholic leaf extracts of
S. mombin and Geraniin are presented in the supplementary data
(S#). The supplementary Table S1, complimented with Figure C 1,
revealed 51 phytochemical compounds from crude ethanolic S.
mombin leaf extract, which is in contrast with reports by Akanji and
co-workers (Akanji, 2020), that identified
25 compounds in SME crude leaf extract. The difference, in conclusion,
maybe due to a possible effect of the geographical location of the
medicinal plant (Behdad et al., 2020).
Geranyl benzoate, as presented in the chromatogram in Figure C 2, was
identified in the crude ethanolic extract of S. mombin leaf
extract. Hence, Geranyl benzoate
(1.06 %) is reported for the first time in ethanolic S. mombinleaf extract, although it has been reported to have been identified as
one of the compounds found in chestnut and highland honey (station 11)
(Kanbur et al., 2021).
Geraniin was reported to have been isolated from ethanolic leaf extract
of S. mombin (Corthout et al.,
1991), but was not identified in the GC-MS analysis of our current
study, partially due to the method of identification.
The GC-MS analysis as presented in Supplementary Tables S3, S4 and S5.
are phytochemicals identified from fractionated ethanolic leaf extract
of S. mombin with 78, 52 and 12 compounds in SMEH, SMEDCM and
SMEEA, respectively. There were three compounds identified to have the
highest compositions in SMEH; Toluene (9.56%), n-Nonadecanol-1 (7.46%)
and Tetradecane (5.23%). Pharmacologically, Tetradecane is reported to
possess anti-microbial, anti-pyretic, anti-helmintic and Tuberculosis
effects (Diwan and Malpathak, 2011).
Similarly, in SMEDCM, Benzaldehyde, 2-chloro- (9.51%), l-(+)-Ascorbic
acid 2,6-dihexadecanoate (7.67%) and Heptadecane (6.30%) were
identified to have the highest compositions. Out of these three
compounds identified, l-(+)-Ascorbic acid 2,6-dihexadecanoate and
Heptadecane were reported by other researchers to possess anti-oxidant
and anti-inflammatory (Shyamala and
Manikandan, 2019, Dulara et al., 2019).
The SMEEA fraction contained Phytol, acetate (28.48%),
3,7,11,15-Tetramethyl-2-hexadecen-1-ol (20.45%) and Eicosane (19.00%)
as the highest percentage compositions. Eicosane has anti-oxidant and
anti-inflammatory effects (Godara et al.,
2019, Dulara et al., 2019).
Similarly, by GC-MS analysis of fractionated methanolic leaf extracts ofS. mombin (SMM) as presented in S5, S6, S7 and S8. The extracts
SMMH, SMMDCM and SMMEA showed 55, 45 and 40 compounds identified,
respectively. The highest percentage composition in the fraction of SMMH
is, n-Nonadecanol-1 (18.98%), Toluene (11.46%) and 1-Butene,
2,3,3-trimethyl (5.51%). Similarly, SMMDCM fraction had Diisooctyl
phthalate (25.74%), Tetrachloroethylene (23.31%) and Eicosane
(16.68%), while SMMEA fraction had, Eicosane (29.23%), Diethyl
Phthalate (10.79 %) and 1-Heptacosanol (9.88%). 1-Heptacosanol , has
anti-bacterial, anti-oxidant and nematicidal effect
(Sultana et al., 2010,
Murugan and Iyer, 2014).