2.4 Fractionation of alcoholic crude leaf extract.
All crude extracts and fractions were pooled together, concentrated
using a rotatory evaporator to reduce the solvent of extraction. They
were then placed in a fume hood at room temperature for several hours
till they were completely dry.
To mimic the ethnomedicinal use of leaf extracts of S. mombin ,
dried ethanolic and methanolic crude leaf extracts of S. mombinwere chosen for fractionation and further analysis
(Cos et al., 2006). They were dissolved
in 50ml of 50% ethanol solution and transferred into a separating
funnel. Liquid-liquid extraction was performed using 100ml, per
extraction solvents of n-hexane, Dichloromethane-DCM and Ethyl acetate.
Each extraction was repeated four times. The fractions were stored in an
airtight glass container and stored in a refrigerator for further use.