2.7.1 System preparation
The x-ray crystal structure of the Marburg virus (MARV) VP35 crystal
structure was retrieved from the Protein Data Bank
(Berman et al., 2002) with code 4GHA
(Bale et al., 2012). The retrieved x-ray
crystal structure consisted of four chains forming a tetramer and was
complexed with the nucleic acid. We deleted all chains, water molecules
and nucleic acids except chain A in preparation for molecular docking
using UCSF Chimera (Pettersen et al.,
2004). Hydrogen atoms were subsequently added and the structure was
saved for molecular docking using AutoDock Vina
(Trott and Olson, 2010).