2.2 Material and Chemical
All chemicals and solvents utilised in this research work were of
analytical reagent grade (AR grade). Geraniin (with certificate of
analysis), Dichloromethane methanol and ethanol were purchased from
Sigma Aldrich and Merck Millipore, South Africa. Hexane and ethyl
acetate were purchased from Associated Chemical Enterprises., South
Africa. Whatman filter paper was purchased from Sigma Aldrich, South
Africa. Different instruments such as the Rotary evaporator and Soxhlet
apparatus (Büchi-Germany), Analytical balance
(Lasec, South Africa) were used in
the study.
The GC-MS instrument used is Shimadzu GCMS2010-SE with a Zebron
ZB-5MSplus 0,25 um, 30 m x 0,25 mm column.