2.2 Material and Chemical
All chemicals and solvents utilised in this research work were of analytical reagent grade (AR grade). Geraniin (with certificate of analysis), Dichloromethane methanol and ethanol were purchased from Sigma Aldrich and Merck Millipore, South Africa. Hexane and ethyl acetate were purchased from Associated Chemical Enterprises., South Africa. Whatman filter paper was purchased from Sigma Aldrich, South Africa. Different instruments such as the Rotary evaporator and Soxhlet apparatus (Büchi-Germany), Analytical balance (Lasec, South Africa) were used in the study.
The GC-MS instrument used is Shimadzu GCMS2010-SE with a Zebron ZB-5MSplus 0,25 um, 30 m x 0,25 mm column.