2.6 Confocal laser scanning microscopy (CLSM)
S. aureus was cultured on glass slides in 6-well plates. Then, it
was stained with four fluorescent dyes, namely, Calcofluor White
(Sigma), Congo Red (40 μg mL–1, Sigma-Aldrich), SYTO
9 (Thermo Fisher) and propidium iodide (PI; Thermo Fisher). Samples were
stained for 30 min and washed with sterile PBS buffer three times.
Finally, 0.5 M carbonate buffer solution mixed with an equal volume of
glycerol was used to fix cells. The cells were observed with an Olympus
FV3000 confocal laser scanning microscope.