Figure 2. Characterization of the luciferase activity and phenotypes of complemented plants.
(a) The T-DNA insertion lines, SALK_047887 and SALK_083115contain insertions within the 3’ UTR and exon 1 of the AT5G08670 , respectively. Transcription proceeds from left to right. (b) Relative expression level of LHCB1.2 in mutants and WT plants after LIN treatment. (c) Relative expression level of LHCB1.2 in WT and mutant plants after NF treatment.
Figure 3. Subcellular localization of AT5G08670 and phenotypic analysis of complemented plants. Subcellular localization of AT5G08670-GFP fusion proteins in Arabidopsis protoplasts. Scale bar, 10 µm.
Figure 4. ATP synthase b-subunit and total ATP synthase activity are decreased in the AT5G08670 mutant. (a) Immunoblotting of mitochondrial ATP synthase - subunit in WT and AT5G08670 mutant. (b) Activity of ATP synthase in WT and AT5G08670 mutant.
Figure 5. Analysis of DEGs after LIN treatment.
(a) Principal component analysis of gene expression in the experimental and control groups. (b) Volcano plot of DEGs (non-significantly different gene expression in grey, significantly different up-regulated and down-regulated genes in red and green; log2 FoldChange (FC)>1, P -value<0.05). (c) Statistics on the number of DEGs after LIN treatment. (d) Heatmap showing the expression patterns of DEGs in different comparison groups (P-value<0.05 and |log2FC|>1).
Figure 6. GO enrichment analysis after LIN treatment.
(a) Venn diagram analysis of significantly down-regulated genes after LIN treatment. (b) GO enrichment analysis of down-regulated genes in mutants after LIN treatment. (c) Venn diagram analysis of significantly up-regulated genes after LIN treatment. (d) GO enrichment analysis of up-regulated genes in mutants after LIN treatment. (e) GO enrichment analysis of significantly up-regulated genes in WT after LIN treatment.