Total protein extraction and immunoblot analysis
The leaves of seedlings were harvested, and total protein was prepared
following the methods of Sun et al. (2016). For immunoblot analyses, the
proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE) (15% acrylamide) (Schägger and von Jagow,
1987). Proteins were then transferred to polyvinylidene difluoride
membranes (Ihnatowicz et al., 2004) and probed with their corresponding
antibodies. Enhanced chemiluminescence was used for signal detection (GE
Healthcare Biosciences).