Plant materials and growth conditions
All Arabidopsis thaliana used in this study were in the Columbia
(Col-0) ecotype background and were obtained from the Arabidopsis
Biological Resource Center. Homozygote mutants were identified by PCR
with corresponding primers. All mutants and WT Arabidopsis were
grown in an artificial climate chamber with the following growth
conditions: 21–23℃, 100 μmol photons
m-2s-1, 18-h light/8-h dark cycle,
and 60–70% humidity. For the NF and LIN treatments, surface-sterilized
mutant and WT seeds were planted on 1/2 Murashige and Skoog (1/2 MS)
medium (PhytoTechnology Laboratories, LLC™, USA) containing 1% sucrose
and 0.8% agar supplemented with either 5 µM NF (Sandoz Pharmaceuticals;
Vienna, Austria) or 220 µg/mL LIN (Sigma; St. Louis, MO).