Vaginal microbiome diversity and composition analysis
Alpha diversity indicators, including the Chao1 richness estimator, Shannon Wiener diversity index, and Simpson diversity index were determined using the ‘vegan’ R package (v2.5). The correlation between alpha-diversity and other variables were evaluated using Spearman’s rank correlation. The p-value was adjusted for multiple tests using the Benjamin – Hochberg method22 , which is controlled by the False Discovery Rate (FDR). FDR values of <0.2 were considered to indicate statistical significance. The overall microbiota composition was analyzed based on Bray Curtis dissimilarity.