Figure 6. Relative transcript levels of miR1885 and its targets
in transgenic and wild-type plants with or without cold treatment.
(A-C) qRT-PCR analysis of RNA levels of miR1885 (A ),Bn.TNL.A03 (B) and Bn.TIR.A09 (C),Bn.CBF1.C03 (D) , Bn.CBF2.Ann (E) andBn.COR15.A03 (F) in transgenic plants after 4 °C cold
treatment for 30 days. Error bar represents mean ± SD; n = 3. *
indicates P < 0.05 and ** indicates P< 0.01 under normal condition; † indicates P< 0.05 and †† indicates P < 0.01 under 4 °C.
ns indicates no significance. Student’s t-test. U6 andACT7 were used as endogenous reference gene for checking miR1885
and targets expression levels, respectively.
Figure 7. Effects of overexpressing and knocking down of miR1885
in B. napus after cold treatment. (A ) Overexpression of
miR1885 increased the sensitivity of cold in B. napus. The cold
treatment was 2 °C for 7 days. (B ) Expression of miR1885 and
its targets in miR1885 knock down plants (STTM1885). Error bar
represents mean ± SD; n = 3. * indicates P <
0.05; ** indicates P < 0.01; Student’s t-test.U6 and ACT7 were used as endogenous reference gene for
measuring miR1885 and targets expression levels, respectively.
(C ) Knocking down of miR1885 increased the resistance to cold
in B. napus as compared to its background. The cold treatment was
2 °C for 14 days. Red scale bar was 5 cm.