Figure S6. Freezing sensitivity of miR1885 over-expressing
transgenic plants.
(A) Phenotypes of transgenic lines grown at 22 °C and −10 °C.
Plants were grown at 22 °C for 6 weeks (upper panel), and then
transferred to −10 °C for 2.5 h (middle panel), and then allowed to
recover at 22 °C for 7 days. (B) Real-time PCR showing the
expression pattern of miR1885 under freezing treatment. U6 was
used as endogenous reference gene. Error bar represents mean ± SD;n = 3. (C) Survival rates of transgenic lines and
wild-type plants after freezing treatment (−10 °C in a growth chamber
for 2.5 h, n = 15–20 plants). * indicates P <
0.05; ** indicates P < 0.01 (Student’s t -test).(D) Proline content in transgenic plants and wild type after
freezing treatment. Error bar represents mean ± SD. * indicatesP < 0.05, significant difference from plants subjected
to −10 °C before freezing treatment (0 h). † indicates P< 0.05, significant difference from plants subjected to −10 °C
for 2.5 h (Student’s t -test).