Figure 3. Genome-wide association study of low-temperature
resistance in a panel of B. napus accessions.
(A) Manhattan plots and Q-Q plots for genome-wide association
study of low temperature resistance phenotype measured in Year 2017
(top) and Year 2018 (bottom), respectively. (B) qRT-PCR
analysis of the abundance of three candidate miRNAs after cold treatment
for 0, 10, 20 and 30 days, respectively. Error bar represents mean ± SE;n = 3. U6 was used as the endogenous reference gene. *
indicates P < 0.05; ** indicates P <
0.01; Student’s t-test. (C ) Haplotypes of candidate SNPs withinMIR1885 promoter for low-temperature resistance in B.
napus population. LTR represents low-temperature element (CCGAAA). Five
variants, including four SNPs and the one-bp deletion, which were
significantly associated with low-temperature resistance, were marked
with dark bold vertical lines. The five variants located in the 200-bp
genomic region around LTR were used for analysis. ‘Hap.’ denotes the
haplotypes of MIR1885 ; ‘Number’ denotes the number of lines
belonging to each haplotype group. ‘Degree’ denotes the average grade of
low-temperature resistance.