Figure 2. Abundance of differentially expressed miRNAs between cold-stress and normal conditions.
(A) qRT-PCR analysis of seven differentially expressed miRNAs in B_V0 and B_V7 libraries. (B) Relative abundance of three differentially expressed miRNAs under normal condition and cold condition detected by northern blotting. (C) Relative abundance of miR1885 in leaf (top) and SAM (bottom) of B. rapa plants after cold treatment for 0, 7, 14, 21 and 28 days, respectively. SAM: shoot apical meristem. “D” represents days. Error bar represents mean ± SE;n = 3. U6 was used as the endogenous reference gene in expression analyses. * indicates P < 0.05; ** indicatesP < 0.01; Student’s t -test.