T cell responses by disease category
IFN-γ+ and IL-2+CD4+ and CD8+ T cell responses were examined by intracellular cytokine staining after stimulation with SARS-CoV-2 S peptide pool (and N and M peptide pools for CoronaVac recipients) as primary cellular outcomes, as tabulated in Table 3. We analyzed S-specific IFN-γ+ CD4+ and CD8+ T cell responses at post-dose 2 and post-dose 3 timepoints by disease category in Fig. 3A-D; results for S-specific IL-2+ CD4+ and CD8+ T cell responses are shown in the Supp. Fig 1A-D. When all IEI patients were considered, 48% and 30% patients respectively had a positive S-specific IFN-γ+CD4+ and CD8+ T cell after 2 doses, with low geometric mean frequencies of 0.016% and 0.007% (Fig. 3A-B). As T cell responses after vaccine were also variable in the healthy population, we were not powered to interpret T cell findings in disease categories with very small sample sizes. Patients with humoral deficiencies mounted a robust T cell response with a geometric mean frequency of 0.044% and 0.01% S-specific IFN-γ+CD4+ and CD8+ T cell responses at the post-dose 2 timepoint. At the post-dose 3 timepoint, 62% and 33% of all patients showed a positive S-specific IFN-γ+CD4+ and CD8+ T cell responses respectively. Patients with humoral deficiencies had S-specific IFN-γ+ CD4+ and CD8+ T cell responses of 0.036% and 0.016%, respectively, at the post-dose 3 timepoint.