T cell responses by disease category
IFN-γ+ and IL-2+CD4+ and CD8+ T cell responses were
examined by intracellular cytokine staining after stimulation with
SARS-CoV-2 S peptide pool (and N and M peptide pools for CoronaVac
recipients) as primary cellular outcomes, as tabulated in Table 3. We
analyzed S-specific IFN-γ+ CD4+ and
CD8+ T cell responses at post-dose 2 and post-dose 3
timepoints by disease category in Fig. 3A-D; results for S-specific
IL-2+ CD4+ and
CD8+ T cell responses are shown in the Supp. Fig 1A-D.
When all IEI patients were considered, 48% and 30% patients
respectively had a positive S-specific IFN-γ+CD4+ and CD8+ T cell after 2 doses,
with low geometric mean frequencies of 0.016% and 0.007% (Fig. 3A-B).
As T cell responses after vaccine were also variable in the healthy
population, we were not powered to interpret T cell findings in disease
categories with very small sample sizes. Patients with humoral
deficiencies mounted a robust T cell response with a geometric mean
frequency of 0.044% and 0.01% S-specific IFN-γ+CD4+ and CD8+ T cell responses at
the post-dose 2 timepoint. At the post-dose 3 timepoint, 62% and 33%
of all patients showed a positive S-specific IFN-γ+CD4+ and CD8+ T cell responses
respectively. Patients with humoral deficiencies had S-specific
IFN-γ+ CD4+ and
CD8+ T cell responses of 0.036% and 0.016%,
respectively, at the post-dose 3 timepoint.