3.1. Metabarcoding data
The ITS1 dataset generated 5,793,980 raw sequence reads, resulting in
5,458,895 gene quality-filtered reads, ranging from 1,252 up to 540,803
per sample. After singletons and rare taxa (<5 reads) removal
(1,108 out of 10,595 ASVs total), a total of 9,487 high-quality ASVs
were obtained. A total of 5,453,881 raw reads were generated from 16S
rDNA dataset and accounted for a total of 4,806,902, which were grouped
into 47,367 ASVs (out of a total of 65,037 ASVs) after quality
filtering, with sequencing depths between samples ranging from 2066 to
265,442 reads.
The microbial community was dominated by fungi (51% of the entire
datasets), where the phylum Ascomycota dominated (72%), followed by
Basidiomycota (15%). Bacteria dominated the prokaryotic diversity,
among which the most abundant phyla were Proteobacteria (40%),
Acidobacteria (16%), and Actinobacteria (14%). The domain Archaea was
much less represented, encompassing only the 2% of ASVs across the
dataset, being constituted mainly by the phylum Thaumarchaeota (62.5%),
while Euryarchaeota and Crenarchaeota were present at lower percentage
(22.2 and 12.4%, respectively).