3.1. Metabarcoding data
The ITS1 dataset generated 5,793,980 raw sequence reads, resulting in 5,458,895 gene quality-filtered reads, ranging from 1,252 up to 540,803 per sample. After singletons and rare taxa (<5 reads) removal (1,108 out of 10,595 ASVs total), a total of 9,487 high-quality ASVs were obtained. A total of 5,453,881 raw reads were generated from 16S rDNA dataset and accounted for a total of 4,806,902, which were grouped into 47,367 ASVs (out of a total of 65,037 ASVs) after quality filtering, with sequencing depths between samples ranging from 2066 to 265,442 reads.
The microbial community was dominated by fungi (51% of the entire datasets), where the phylum Ascomycota dominated (72%), followed by Basidiomycota (15%). Bacteria dominated the prokaryotic diversity, among which the most abundant phyla were Proteobacteria (40%), Acidobacteria (16%), and Actinobacteria (14%). The domain Archaea was much less represented, encompassing only the 2% of ASVs across the dataset, being constituted mainly by the phylum Thaumarchaeota (62.5%), while Euryarchaeota and Crenarchaeota were present at lower percentage (22.2 and 12.4%, respectively).