2.3.6. Triacylglycerols analysis
Triacylglycerol analysis was carried out by modifying the COI method
(2017) and the method used by Louati et al. (2014) [15, 17]. Agilent
Infinity II 1260 HPLC device with a reverse phase column and refractive
index detector (RID) was used. Triacylglycerols were separated from
other oil components by column chromatography. The oil sample dissolved
in petroleum ether was placed on a previously conditioned chromatography
column containing a silica gel absorber. An ACE 5 C18 column (250 mm x
4.6 mm x 5 µm) was used for separation. Chromatography conditions;
mobile phase acetonitrile/acetone (36.5:63.5), column temperature 35°C,
flow rate 1.0 mL/min and injection volume of samples 20 µL.
Triacylglycerols were identified by comparison with a reference
chromatogram [18, 19].