2.3.1. Oil extraction
A cold press oil machine (Karaerler brand NF500 model, Turkey) was used to extract the oil from pine nuts. The pine nuts were pressed at a frequency of 15 Hz at 45oC. The cold press oil machine was preheated and the temperature was set to 100 °C. The amount of cold pressed pine nut oil (%) was determined from the ratio of the amount of pine nut that was initially cold pressed and the amount of oil obtained as a result of the process. After filtering the pine nut oil, it was stored in dark bottles at 4 degrees under refrigerator conditions.