2.3.6. Triacylglycerols analysis
Triacylglycerol analysis was carried out by modifying the COI method (2017) and the method used by Louati et al. (2014) [15, 17]. Agilent Infinity II 1260 HPLC device with a reverse phase column and refractive index detector (RID) was used. Triacylglycerols were separated from other oil components by column chromatography. The oil sample dissolved in petroleum ether was placed on a previously conditioned chromatography column containing a silica gel absorber. An ACE 5 C18 column (250 mm x 4.6 mm x 5 µm) was used for separation. Chromatography conditions; mobile phase acetonitrile/acetone (36.5:63.5), column temperature 35°C, flow rate 1.0 mL/min and injection volume of samples 20 µL. Triacylglycerols were identified by comparison with a reference chromatogram [18, 19].