2.3.5. Fatty acids analysis
Determination of the fatty acid composition of pine nut oil was carried out by modifying TS EN ISO 12966-2 and Method 4 methods [15, 16]. The pine nut oil was extracted and fatty acid methyl esters (FAME) were prepared. After derivatization, they were injected to a GC instrument with a flame ionization detector (Agilent 7820A GC-FID) and a capillary column (HP-88 column; 100 m x 250 µm i.d. x 0.25 µm film). Injector temperature of 250 °C, detector temperature of 280 °C and helium (1.0 mL/min) as carrier gas were used as chromatographic conditions. The amounts of individual fatty acids were determined by comparison with the retention times of the standard solutions and expressed as a relative percentage.