Declarations:
Ethics approval and consent to participate: Not applicable
Consent for publication: Not applicable
Availability of data and materials : The sequencing datasets supporting the conclusions of this article are available under the NCBI SRA: SUB11545621. Additional Data including GCxGC data can be found in Supplemental Materials file.
Competing interests : The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Funding: This project was financially supported by the Pioneer Oil Company (Vincennes, IN) and the Purdue Enhanced Oil Recovery Lab.
Author Contributions: ETH: Conceptualization, Formal analysis, Data curation, Investigation, Methodology, Project administration, Supervision, Validation, Visualization; Roles/Writing - original draft. LECM: Formal analysis, Methodology, Data curation, Investigation, Writing - review & editing. GK: Funding acquisition, Project administration, Resources, Writing - review & editing. KVS: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing - review & editing. All authors have read and agreed to the published version of the manuscript.
Acknowledgments: We would like to thank the members of Purdue’s Enhance Oil Recovery Lab, specifically Dr. Nate Schultheiss, Dr. Thomas Everett, Dr. Jeremy Holtsclaw, Dr. Katherine Wehde, and Timothy Henderson, who made this work possible by coordinating well samplings, provided experimental feedback, and supplying technical supported and insight for our work. We would also like to thank Dr. Stephen Lindemann and his lab at Purdue Food Science for providing access to their Micro GC for H2S analyses. We are also grateful to the Purdue University Genomics Core for their role in sequencing the 16S rRNA gene amplicons and the Purdue Institute of Inflammation, Immunology, and Infectious Disease for access to the Bell computing cluster which allowed us to analyze the sequencing reads. Additionally, we are thankful to both Bindley Bioscience Center and the Fuel Laboratory of Renewable Energy at Purdue University for the research space and support staff where this research was carried out.