Statistical analysis
All statistical analyses were performed using the SPSS 25.0 software. Categorical data were expressed as percentages and values, and numerical data with a normal distribution were expressed as mean±standard deviation. Otherwise, median and interquartile range were used to express data with a skewed distribution. Quantitative data were compared using t-test. and analysis of variance (ANOVA) was performed for multiple comparisons among groups with post hoc tests. A two-sided test was used, and statistical significance was set at P<0.05.