Cell Viability Assay
The cell viability was determined by Cell Counting Kit-8 (CCK8)
according to the manufacturer’s protocol (Cat#C0043, Beyotime, China).
HaCaT cells (1×104 cells/200μl) were seeded into 96-well plates
overnight and incubated with different concentrations of allicin for 48
h. After adding 20μl of CCK-8 solution to each well for 1 h at 37°C, the
optical density (OD) was measured using a multifunctional microplate
reader (2300, PerkinElmerEnSpire) at 450 nm.