Validation by RT-qPCR analysis
To verify the accuracy of the RNA-Seq data, 8 olfactory- and
chemosensory-related genes were selected from among the DEGs and
validated by RT-qPCR analysis. All selected DEGs were significantly
expressed in the antennae. The results of RT-qPCR were consistent with
those of RNA-Seq, indicating the reliability of the RNA-Seq data (Figure
8 and Table S1).