Figure 6 KEGG enrichment analysis for unigenes. The concrete description for the KEGG pathways (level 2) is listed on the left; the number at the end of each bar chart indicates the number of annotated unigenes. The descriptions of six major categories of KEEG terms (level 1) are listed on the right. The lines at the top of the figure show the KEGG level as well.
Identification and annotation analysis of DEGs
Based on the DESeq2 software, a total of 4889 DEGs with the FDR < 0.01 and | log2 (Fold Change) |≥ 2 were identified between MP and NMP groups, including 2128 upregulated and 2761 downregulated unigenes (Figure S2). Furthermore, the annotations from the GO analysis of DEGs were classified into 44 GO categories, including the BP (18), CC (14), and MF (12). The top 3 GO terms in CC were cell part (GO:0044464), membrane part (GO:0044425), and protein-containing complex (GO:0032991); in BP were, cellular process (GO:0009987), metabolic process (GO:0008152), and biological regulation (GO:0065007); in MF were, binding (GO:0005488), catalytic activity (GO:0003824), and structural molecule activity (GO:0005198) (Figure 7).