The tropical calanoid Pseudodiaptomus incisus
Pseudodiaptomus incisus were collected from a coastal aquaculture pond (11.82397°N, 109.1233°E) in Cam Ranh Bay, July 2020, using a zooplankton net (mesh size = 200 μm). Copepods were transferred to the Copepod Laboratory at Cam Ranh Centre for Tropical Marine Research and Aquaculture, Nha Trang University. The pond water salinity and temperature were 27 - 28°C and 35 PSU, respectively. Healthy adult copepods (F0) were sorted and then divided into 5-L bottles, approximately 1200 individuals per bottle, for acclimation. Adult males and females were acclimated in water baths to 30°C or 34°C for three days. The temperature was increased by 1°C every 12 hours until reaching the experimental temperatures. During the acclimation, the salinity, light : dark cycle, and dissolved oxygen concentration were kept at 30 PSU, 12L:12D, and >5 mg L-1 by aerations, respectively (see also Truong et al., 2020). P. incicus were fed two times a day with Isochrysis galbana at 30,000-33,000 cell L-1 (~800 – 850 μg carbon per litter, Doan et al., 2018).