The tropical calanoid Pseudodiaptomus incisus
Pseudodiaptomus incisus were collected from a coastal aquaculture
pond (11.82397°N, 109.1233°E) in Cam Ranh Bay, July 2020, using a
zooplankton net (mesh size = 200 μm). Copepods were transferred to the
Copepod Laboratory at Cam Ranh Centre for Tropical Marine Research and
Aquaculture, Nha Trang University. The pond water salinity and
temperature were 27 - 28°C and 35 PSU, respectively. Healthy adult
copepods (F0) were sorted and then divided into 5-L bottles,
approximately 1200 individuals per bottle, for acclimation. Adult males
and females were acclimated in water baths to 30°C or 34°C for three
days. The temperature was increased by 1°C every 12 hours until reaching
the experimental temperatures. During the acclimation, the salinity,
light : dark cycle, and dissolved oxygen concentration were kept at 30
PSU, 12L:12D, and >5 mg L-1 by aerations,
respectively (see also Truong et al., 2020). P. incicus were fed
two times a day with Isochrysis galbana at 30,000-33,000 cell
L-1 (~800 – 850 μg carbon per litter,
Doan et al., 2018).