Cumulative nauplii and faecals
To evaluate the reproductive output and the grazing rate of P.
incisus , we quantified the cumulative nauplii and faecals in five days.
Ten adults of both sexes were transferred to a separated 1-L bottle (10
replicates per treatment). Bottles were daily filtered using the same
filtering net (see above) to collect the nauplii and faecals. Alive
adults were returned to the bottle while the dead ones were removed. The
content containing nauplii and faecals was transferred to a petri dish,
fixed with Lugol (4%). The number of nauplii and feacals was counted
using a stereo-microscope (SZ51, Olympus, Japan).