Preparation of Single Cell Suspension
Cells were transported in DMEM (ThermoFisher Scientific, Waltham, MA) on
ice immediately after above procurement. This suspension was then
filtered using a 40µm strainer (ThermoFisher Scientific) and residual
cell clumps were discarded. The suspension was immediately placed on
ice. After centrifuging at 300g at 4°C for 6 minutes, the supernatant
was discarded and the cell pellet was re-suspended in DMEM and placed on
ice prior to staining for loading.