Preparation
The provocation test is performed at the temperature of 14-20℃ in a quiet room which is equipped with first-aid measures such as epinephrine, dexamethasone, antihistamines, tracheotomy packages, and oxygen inhalation devices. We first prepared well the solvent solution, allergen solution (HDM 1.0mg/ml, Wolwobiotech, China) in two different marker provocation device (the sterile syringe). Then the patient’s scores of total nasal symptom scores (TNSS) , visual analog scale (VAS) and anterior nasal endoscope and nasal flow test were assessed at baseline and after nasal provocation.