Sample collection and analysis
On day 7 post-stress induction or in NST group, mice were anesthetized
with ketamine–xylazine (100:20) intraperitoneal (i.p) plus maintenance
dose of isoflurane (2.5%) and euthanized by cardiac puncture. The
plasma was collected to determine the levels of corticosterone,
cytokines, and CGRP.