Statistical analysis
Data were presented as mean ± standard error of mean (S.E.M.) and
statistically analyzed by parametric and nonparametric Student’s t-test,
one-way ANOVA, repeated measures, and two-way mixed model analysis of
Variance (ANOVA) according to the experimental protocol. First to
determine the presence of an interaction effect, and then to compare the
NST or vehicle control and treated groups in both sex of mice at each
time point tested. The post hoc comparisons employed the Bonferroni
criterion. p values less than 0.05 were considered significant.
The Imax was calculated using the following formula: 100 x (h
post-treatment – mean of basal post-induction)/(basal post-induction
mean – basal post-induction mean). Statistical analyses were performed
using Graph Pad Prism 9.0 software.