Behavioral experiments
Firstly, baseline grimace scale, periorbital and hind paw thresholds to
von Frey filaments were recorded for each animal. Once periorbital
thresholds had been tested, animals were placed back in hind paw testing
chambers to habituate for approximately 5 minutes before beginning hind
paw testing [10]. After, post-stress induction the behavioral
experiments were evaluated on days 1, 3, 7, 10, and 14 days.
Subsequently, on the nociceptive peak after stress induction (7 days)
the treatment protocol with CGRP antagonist or the different plasma or
trigeminal ganglion analyses were performed.