Behavioral experiments
Firstly, baseline grimace scale, periorbital and hind paw thresholds to von Frey filaments were recorded for each animal. Once periorbital thresholds had been tested, animals were placed back in hind paw testing chambers to habituate for approximately 5 minutes before beginning hind paw testing [10]. After, post-stress induction the behavioral experiments were evaluated on days 1, 3, 7, 10, and 14 days. Subsequently, on the nociceptive peak after stress induction (7 days) the treatment protocol with CGRP antagonist or the different plasma or trigeminal ganglion analyses were performed.