Statistical analysis
Data were presented as mean ± standard error of mean (S.E.M.) and statistically analyzed by parametric and nonparametric Student’s t-test, one-way ANOVA, repeated measures, and two-way mixed model analysis of Variance (ANOVA) according to the experimental protocol. First to determine the presence of an interaction effect, and then to compare the NST or vehicle control and treated groups in both sex of mice at each time point tested. The post hoc comparisons employed the Bonferroni criterion. p  values less than 0.05 were considered significant. The Imax was calculated using the following formula: 100 x (h post-treatment – mean of basal post-induction)/(basal post-induction mean – basal post-induction mean). Statistical analyses were performed using Graph Pad Prism 9.0 software.