Alignment
Data analysis was performed using the Torrent Suite Software v5.0.4
(Life Technologies). Reads were aligned to the hg19 human reference
genome from the UCSC Genome Browser (http://genome.ucsc.edu/) and to the
BED file designed using Ion AmpliSeq Designer. Alignments were visually
verified with the software Alamut® v2.13 (Interactive Bio software).