Seed surface sterilization
Seeds of Arabidopsis and Artemisia were surface sterilized as previously
described by (van de Mortel et al. 2012). Briefly, the seeds were
placed in opened Eppendorf tubes inside a desiccator with two 100-mL
beakers each containing 50 mL sodium hypochlorite (12% v/v). To each of
the beakers, 1.5 mL concentrated HCl was added and the seeds were
exposed for three hours to the chlorine gas generated inside the
desiccator. Seeds of Brassica olearacea var. italica, cultivar
Coronado were surface sterilized for 30 min in 1% (v/v) sodium
hypochlorite supplemented with 0.1 % (v/v) Tween 20 and then washed
three times with ample sterile distilled water.