Bacterial strains and culture conditions
Pseudomonas fluorescens SS101 (Pf SS101) was cultured in
King’s medium B (KB), while Microbacterium EC8 (MB) was cultured
in Tryptone Soy broth (TSB) medium and Paraburkholderia graminisPHS1 (Pbg ) in Luria Bertani (LB) medium (Lennox, Carl Roth) at 25
°C for 16h. Bacterial cells were collected by centrifugation, washed
three times with 10 mM MgSO4, and resuspended in 10 mM
MgSO4 to a final density of
~109 cells/ml (OD600 =
1.0).