DNA extraction and whole-genome sequencing
DNA from saliva and FFPE samples was purified and extracted using Qiagen Kit (Qiagen Systems) and Promega Kit, respectively. WGS libraries were prepared for Illumina 150bp paired-end reads sequencing using the NEBNext Ultra II DNA Library Prep Kit protocols. All libraries were sequenced on the Novaseq 6000 platform (Illumina, San Diego, CA, USA) using standard protocols. Whole-genome analysis was performed by the Utah Center for Genomic Discovery (UCGD) at the University of Utah. Germline SNVs and SVs for each sample (22 samples total) were detected following a Genome Analysis Tool Kit (GATK) best practices equivalent workflow for variant detection.18