2.6 Effects of mutations on IRX4 protein
I-mutant server was used to calculate the stability of the WT protein
and mutants 48. Default
parameters of the server such as pH value of 7 and temperature 25
were used for this analysis. The stability of the WT and mutant protein
structures were calculated based on amino acid properties, relative
solvent accessibility surface, evolution and structural information of
the protein 49.