Additionally, we also checked the ability of the mutant protein to be a cell-penetrating peptide sequence. As depicted in Table 7, the mutation of Arginines on the C- and N-terminal of the homeodomain leads to a decrease in confidence of the peptide to cell penetrating. As previously mentioned, cellular uptake peptides is increased due to multiple arginines by attaching fatty acid to N-terminal of the peptide⁸⁵. It has also been mentioned that the presence of more than six arginine residues is critical for efficient cell-penetrating functions⁸⁵. The ability of these peptides for convenient cellular uptake needs to be studied in detail to find their efficacy as therapeutic agents targeting IRX homeodomain family in different cancers.