Characterization of Iceland IAV isolates
Throughout 2010-2018, 6635 waterfowl, seabirds, and shorebirds were sampled (Anseriformes (n = 1216), Charadriiformes (n = 5289), Galliformes (n = 75), Passeriformes (n= 4), Pelicaniformes (n = 39), and Procellariiformes (n = 12)) in 34 unique locations throughout Iceland, in the following regions: Southwest (n = 4747), South (n=664), West (n = 312), East (n = 457), Northeast (n = 450), and Northwest (n=5). Of the total collected, 381 (5.7%) tested positive for IAV by RT-PCR, and 94 IAVs were ultimately isolated and sequenced. Thirty-five of the 94 IAV sequences have been previously described (Dusek et al., 2014; Guan et al., 2019; Hall et al., 2014). The PB2 gene of one isolated IAV failed to be sequenced, therefore 93 IAV sequences were included in this analysis. IAVs isolated in Iceland during the sampling timeframe, included 20 distinct subtypes from 92 individual birds (Supplementary table 1a). H6N8, H16N3, H2N5, and H2N7 subtypes were dominant in the dataset, while others were less frequent. The 92 individual birds included 9 species of the order Charadriiformes, and three species of the order Anseriformes, and ranged in age from 1CY to 4CY+ (Supplementary Table 1b). Sampling was conducted throughout multiple seasons across years, resulting in sequenced viruses from autumn (n=72), spring (n=19), and winter (n=1) months.