3.1.2.4. Chitosan-Based Electrochemical Enzyme Biosensor:
Chitosan appears as a porous polymer for adherence of enzymes by its
polycationic chains to form the biosensor. Cross-linking drugs such as
GL, carbodiimide, NH, epichlorohydrin or GL, NH or cyanuric acid are
suitable for this attachment eg., a cross-link such as GL and GL. The
adhesion does not interfere with enzyme movement and function. The
important aspect in connecting Chitosan with various chemical groups is
the abundance of functional groups and pH solubility. Many applications
of chitosan-based electrochemical enzyme biosensor were reported,
including for dopamine release in the brain of rats (Njagi et al.,2008),
detection of hydrogen peroxide via catalase immobilized on
chitosan-β-cyclodextrin (Dong et al., 2017) and chlorophenol
determination via laccase immobilized on ZnO-Chitosan nanocomposite
(Mendes et al., 2017).