3.1.2.4. Chitosan-Based Electrochemical Enzyme Biosensor:
Chitosan appears as a porous polymer for adherence of enzymes by its polycationic chains to form the biosensor. Cross-linking drugs such as GL, carbodiimide, NH, epichlorohydrin or GL, NH or cyanuric acid are suitable for this attachment eg., a cross-link such as GL and GL. The adhesion does not interfere with enzyme movement and function. The important aspect in connecting Chitosan with various chemical groups is the abundance of functional groups and pH solubility. Many applications of chitosan-based electrochemical enzyme biosensor were reported, including for dopamine release in the brain of rats (Njagi et al.,2008), detection of hydrogen peroxide via catalase immobilized on chitosan-β-cyclodextrin (Dong et al., 2017) and chlorophenol determination via laccase immobilized on ZnO-Chitosan nanocomposite (Mendes et al., 2017).