Target Sequence Reference
Oncorhynchus mykiss
Forward: 5’- AGTCTCTCCCTGTATATCGTC -3’ Reverse: 5’- GATTTAGTTCATGAAGTTGCGAGAGTA -3’ Probe: 5’-[FAM]-CCAACAACTCTTTAACCATC- [NFQ]-[MGB]-3’
The underlined base “A” was modified from (Wilcox et al., 2015)
Plecoglossus altivelis
Forward: 5’-CCTAGTCTCCCTGGCTTTATTCTCT-3’ Reverse: 5’-GTAGAATGGCGTAGGCGAAAA-3’ Probe: 5’-[FAM]-ACTTCACGGCAGCCAACCCCC-[TAMRA]-3’
(Doi et al., 2017)
Cyprinus carpio
Forward: 5’-GGTGGGTTCTCAGTAGACAATGC-3’ Reverse: 5’-GGCGGCAATAACAAATGGTAGT-3’ Probe:5’-[FAM]-CACTAACACGATTCTTCGCATTCCACTTCC-[TAMRA]-3
(Takahara et al., 2012)