2.3 Annotation and comparative analysis of the O. xuefengensis mitogenome
Ab initio prediction was used to generate gene models for the O. xuefengensis mitogenome. Gene models were identified using MFannot (https://megasun.bch.umontreal.ca/cgi-bin/dev_mfa/mfannotInterface.pl). Gene models were then compared against the non-redundant (NR) NCBI database using BLASTp, in addition to comparison against the SwissProt, KEGG, and COG databases to establish functional annotation, also using BLASTp. tRNAs were identified using the tRNAscan-SE program (v1.23, http://lowelab.ucsc.edu/tRNAscan-SE) and rRNAs were identified using the RNAmmer program (v1.2,http://www.cbs.dtu.dk/services/RNAmmer/). Circular maps of the four mitochondrial genomes were drawn using OGDRAW (https://chlorobox.mpimp-golm.mpg.de/OGDraw.htmlonline) [41].