22 Mitochondrial DNA sequencing and genome assembly
About 8 μg of purified DNA was sequenced using a combination of third-generation sequencing technologies including PacBio RS and Illumina sequencing platforms. The Illumina data was used to evaluate the complexity of the genome and correct the PacBio long reads. The raw paired-end reads were trimmed, and quality controlled using Trimmomatic with parameters including SLIDINGWINDOW: 4:15 MINLEN: 75 (version 0.36http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic). Clean data obtained from the above quality control procedures were then used for further analyses.