Figure 5 Anti-inflammatory effect of Cav3.2 channels inhibition is mediated by immune cells
Edema development after carrageenan injection in Cav3.2Nav1.8 cKO and Cav3.2GFP-flox KI mice (A ). (B ) Representation of chimeric mice generation. WT and Cav3.2 KO mice (recipients) are irradiated. In irradiated mice, bone marrow cells retrieved from un-irradiated WT or Cav3.2 KO mice (donors) were transplanted by intravenous injection. Four experimental groups were obtained: WT mice transplanted with bone marrow cells from Cav3.2 KO; Cav3.2 KO mice transferred with bone marrow cells from WT donors; WT mice transferred with bone marrow cells from WT donors; and Cav3.2 KO mice transferred with bone marrow cells from KO donors. Edema development (C ) and IL-6 production (D ) in chimeric mice 4 hours after injection of carrageenan, 8 weeks after the bone marrow transplantation. (E ) BMDM and CD4+ T cells, obtained from WT and Cav3.2GFP-flox KI mice stained with antibody anti-eGFP or isotype control revealing the expression of Cav3.2 in these cells.B was analyzed by one-way ANOVA followed by Bonferroni post-hoc test: no significant differences were found. For other graphs a one-way ANOVA followed by Bonferroni post-hoc test were performed (*p < 0.05; **p< 0.01; ***p < 0.001). Data represent mean ± SEM.