Animals
All experiments were performed in accordance with the ethical guidelines
set out by the International Association for the Study of Pain and the
relevant European legislation (Directive 2010/63/EU) and given approval
by the local ethics committee (CEMEA Auvergne; reference 6295).
Eight-week-old male C57BL/6j mice were purchased from Janvier
Laboratories (Le Genest-Saint-Isle, France). Cav3.2
knock-out (Cav3.2 KO) male mice, originally generated by
Chen et al. (2003), and their wild type (WT) littermates were
bred in the animal facility of Clermont-Auvergne University. We also
used Cav3.2-GFP-flox knock-in
(Cav3.2GFP-flox KI) mice, generated by
Francois et al. (2015). A conditional Cav3.2 KO
mouse in C-LTMRs was generated by crossing the
Cav3.2GFP-flox KI mice with
Nav1.8cre KI mice (Stirling et al.,
2005; François et al., 2015). The resulting mice were designated
Cav3.2Nav1.8 cKO, their control
littermates (Cav3.2GFP-flox KI mice)
were also used. Chimeric mice were generated to determine the role in
inflammation of Cav3.2 located in immune versus neuronal
cells. All animals were housed under standard laboratory conditions
(12-hour light/dark cycle, temperature of 21 to 22°C, 55% humidity
under specific pathogen free conditions). Food and water were availablead libitum . They were acclimatized for a week before testing.