Statistical analysis
The data and statistical analysis comply with recommendations for experimental design and analysis in pharmacology (Curtis et al., 2015, 2018). Statistical analysis was performed with Prism 7 software (GraphPad Prism, La Jolla, USA). All data are expressed as the mean ± standard error of the mean (SEM). We used a two-way (time and pharmacological treatment or time and genotype) analysis of variance (ANOVA) followed by the Bonferroni post-hoc test for multiple comparisons. For other analyses, we used a Mann-Whitney test. Data were considered statistically significant when p was < 0.05.