2.2 Cell treatment
HK-2 cells were cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS) and 100 U/ml penicillin/streptomycin at 37 ℃ and 5% CO2. After being cultured in the absence or presence of different concentrations of leonurine for 1 h, the cells were treated with different concentrations of RSL3 or erastin for 24 h and then collected or stained for subsequent experiments.