2.2 Cell treatment
HK-2 cells were cultured in DMEM/F12 supplemented with 10% fetal bovine
serum (FBS) and 100 U/ml penicillin/streptomycin at 37 ℃ and 5%
CO2. After being cultured in the absence or presence of
different concentrations of leonurine for 1 h, the cells were treated
with different concentrations of RSL3 or erastin for 24 h and then
collected or stained for subsequent experiments.