Peripheral blood karyotype analysis
Chromosome analysis was performed on routinely cultured peripheral blood
lymphocytes as described previously[12]. Briefly,the sections were
treated with trypsin using standard techniques, the slides were Giemsa
stained and then G-banding analysis was performed. Add colchicine 4
hours before cytology preparation. For each sample, at least 20 cells
from two independent cultures were used for microscopic observation and
analysis in metaphase.