Cold stimulation testing and critical temperature measurements
ICT was performed following a standard protocol. Briefly, a melting ice
cube in thin plastic bag was placed on the volar surface of the patient
forearm for 5 min, with macroscopic assessment of the test site 10 min
later. TempTest® CSTs were done with
TempTest® 4.0 (Courage & Khazaka, Cologne, Germany),
which has a single Peltier element (length: 350 mm, width: 2 mm) that
provides a continuous temperature gradient from 4°C to 44°C. The use of
TempTest® allows for reproducible and standardized
cold (and heat) provocation tests and the identification of CTTs.
TempTest® CST results were also assessed 10 min after
the end of cold exposure.