2.9 Identification of genes potentially involved in gall formation and host manipulation
A total of 141 proteins have been identified from saliva of S. chinensis in a previous study (Yang et al., 2018). BLASTX was used to search the corresponding genes in S. chinensis genome with the 141 salivary proteins as queries (E-value ≤1×10−5, identify ≥ 50). The expression of salivary protein-encoding genes was analyzed in three stages based on RNA-seq data. The GO and KEGG enrichment analyses highly expressed genes of fundatrix were conducted using Omicshare CloudTools under this tool’s default instructions (http://www.omicshare.com/).