2.9 Identification of genes potentially involved in gall
formation and host manipulation
A total of 141 proteins have been identified from saliva of S.
chinensis in a previous study (Yang et al., 2018). BLASTX was used to
search the corresponding genes in S. chinensis genome with the
141 salivary proteins as queries (E-value ≤1×10−5, identify ≥ 50). The
expression of salivary protein-encoding genes was analyzed in three
stages based on RNA-seq data. The GO and KEGG enrichment analyses highly
expressed genes of fundatrix were conducted using Omicshare CloudTools
under this tool’s default instructions (http://www.omicshare.com/).