3 Results and Discussion
The four most stable conformations of probe C23 were found as shown in Figure1. The difference among the four conformations were clearly depicted in the front and side view of the molecular structure of the probes due to the rotational bonds. The transfer among the different conformations provided the non-radiation energy loss when the probe C23 recovered back to the ground state from excited states, led to no fluorescence generation with the probe C23 itself. When the probe was incorporated into a hydrophobic cavity of HSA, the bonding interaction between the probe and HSA resulted in the inhibition of non-irradiative energy loss pathways and an increase in fluorescence within the red channel.