Figure 2. a) shows the transconjugants per recipient (T/R) of
the plasmids R27 and pB10 from the E. coli plasmid donor (E. coli/pB10
and E. coli/R27) to the sewage community, in conjugation assays. The
figure summarizes transfer to the sewage community of Ellinge-,
Klagshamn- and Sjölunda WWTP, WWTP are additionally indicated by color.
Six biological replicates were made for each plant per donor/plasmid.Dot plot b) shows the T/R from the sorted and enriched sewage
community, harboring either pB10 (Enriched T. sewage/pB10) or R27
(Enriched T. sewage/R27), to the recipient E. coli strain. Each enriched
transconjugant sewage community sample number is indicated by color, and
conjugation assays were performed as three technical replicates per
enriched transconjugant sample. In comparison plasmid transfer from the
E. coli plasmid donor strain (E. coli/pB10 and E. coli/R27) to the E.
coli recipient strain is shown and color coded gray. For both
plots are donors shown on the x-axis and the y-axis represents T/R,
shown as a logarithmic scale. P values indicate significant differences
(Wilcoxon rank-sum test, adjusted p values) in T/R. Error bars represent
the standard deviation and the black dot the mean.