Haematoxylin and Eosin Staining
After 2 weeks, the rats were perfused with normal saline, followed by
treatment with 4% paraformaldehyde dissolved in 0.1 M PBS to fix the
tissues. Then, the tissue samples were collected and post-fixed at 4°C
for 8 h. The tissue samples were then dehydrated in 20% and 30%
sucrose dissolved in PBS for dehydration, respectively, at 4°C. Next,
10-μm thick slices were subjected to haematoxylin and eosin (H&E)
staining. The tissue samples were photographed with a digital camera
(HUAWEI P40, China), and the samples were examined and imaged using a
high-quality DMI4000 fluorescence microscope (Leica, Germany).