Figure 1: Proportion of surviving individuals after a 4-hour
heat stress. Focal individuals were subjected to a pre-stress
‘hardening’ treatment for 1-hour immediately prior to temperature
stress. a) D. virilis individuals of unknown sex were
heated during the pupal stage and subjected to a range of stressful
temperatures. A range of hardening temperatures were also used to
examine the hardening response. Note that the 34 and 35°C hardening
temperatures were not measured at 37 and 38°C temperature stress.b) Male and female D. virilis were heated during the
adult stage 7 days post-emergence, and subjected to two stress
temperatures (23°C: benign, 38°C, stress). Error bars represent 95%
confidence intervals.