Contiguity, accuracy, and BUSCO results of the aDNA (Takifugu flavidus ) assemblies using the original in silico method (*) and optimized in silico method (**). ‘rub’ short for ‘Takifugu rubripes ’, ‘bim’ short for ‘Takifugu bimaculatus ’. no_ in silico: without in silico method; *: original in silicomethod using one reference; **: optimized in silico method using multiple references; @: Ragoo method using one reference.