Multiple sequence alignment
Multiple alignment of Hsp60 amino acid sequences was performed using the
ClustalOmega software26 and the BioPython 1.68
library27. Multiple alignment of Hsp60 nucleotide
sequences was performed using the MUSCLE program28 and
the MEGA-CC software29. The nucleotide sequences were
aligned using the MUSCLE Codons option. Here, all codons in the
sequences have been converted to amino acids using the standard genetic
code. The obtained amino acid sequences were aligned with subsequent
reverse translation of amino acids into codons. Note that this option is
valid for encoding nucleotide sequences in multiples of three and
without reading frame displacement.