Blood Sampling
After evaluation of the FMD response the routine 10 ml venous blood samples were obtained from each participant. After centrifugation, routine laboratory parameters (white blood cell [WBC] (10-3/uL), hemoglobin (g/dL), platelet (10-3/uL), mean platelet volume [MPV] (fL), creatinine (mg/dL), alanine aminotransferase [ALT] (IU/L), aspartate aminotransferase[AST] (IU/L), D-dimer (ng/L), fibrinogen (μg/ml), albumin (g/dL), fibrinogen to albumin ratio [FAR] (%), Neutrophil to Lymphocyte ratio [NLR] (%), D-dimer to albumin ratio [DDAR] (%)) and monocyte chemo-attractant protein-1 [MCP-1] (pg/ml) levels were studied from obtained serum samples. The commercially available human MCP1 ELISA kit (SUNLONG, Sun Long Biotech Co., LTD) was used for determining MCP-1 levels (SUNLONG, Sun Long Biotech Co., LTD) as described in previous reports 10 .