Figure
4. Residue conservation output for the (A) hs CENP-H model and
the (B) hs CENP-M (PDB 4P0T). The color gradient shows the
different scales of conservation. Highly conserved residues are colored
in deep red while white coloration indicate average conservation.
Non-conserved residues are colored in blue. Based on the neural network
algorithm, “e” and “b” indicate exposed and buried residues
respectively while “f” and “s” respectively denote functional and
structural residues.
In a similar study involving the human CENP-M (PDB 4P0T), conserved
surface residues were also identified to be involved in the interaction
with the C-terminal of the hs CENP-I; an interaction which leads
to the stabilization of the hs CENP-I and likewise required for an
unabated kinetochore localization [37]. Using the same computational
approach, the reported conserved surface residues of the hs CENP-M
were also shown to be conserved and each exhibit varying degrees of
conservation (Figure 4B), hence validating experimental reports from the
previous studies.