Figure 4. Residue conservation output for the (A) hs CENP-H model and the (B) hs CENP-M (PDB 4P0T). The color gradient shows the different scales of conservation. Highly conserved residues are colored in deep red while white coloration indicate average conservation. Non-conserved residues are colored in blue. Based on the neural network algorithm, “e” and “b” indicate exposed and buried residues respectively while “f” and “s” respectively denote functional and structural residues.
In a similar study involving the human CENP-M (PDB 4P0T), conserved surface residues were also identified to be involved in the interaction with the C-terminal of the hs CENP-I; an interaction which leads to the stabilization of the hs CENP-I and likewise required for an unabated kinetochore localization [37]. Using the same computational approach, the reported conserved surface residues of the hs CENP-M were also shown to be conserved and each exhibit varying degrees of conservation (Figure 4B), hence validating experimental reports from the previous studies.