Modeling of the human CENP-H, CENP-I and CENP-K
Structural models were generated using the amino acid sequence of each protein as described in the methods segment, as input. The 3D structure prediction method employed by RaptorX contact is unique in that it makes a simultaneous prediction of all protein contacts, which allows for an easy modeling of high order residue correlation. The output provides 5 different models that are ranked by estimated root mean square deviation (RMSD). The estimated RMSD is a calculated average deviation distance in Å of a 3D model from its experimental structure. The smaller the estimated RMSD value is, the higher the likelihood of the 3D model to a good quality. The estimated RMSD values of the top rank models for CENP-H, -I and -K (Figure 1) are 5.7546 Å, 13.445 Å and 5.7311 Å respectively. All generated models share high similarity with the PDB structures of their respective orthologs (Supplementary Figures S1 and S2) and as such were selected for structural refinement.