Figure 2. Z-score plots for (A) CENP-H, (B) CENP-K and (C) CENP-I. The plots contain the z -scores of all protein chains in the current PDB that has been experimentally determined. In this plot, structural groups from different sources such as, X-ray and NMR ,are differentiated by different colors.
The PROCHECK suite was used for the calculation of the stereochemical quality of the models through the analysis of the overall structural and individual residue geometry. The Ramachandran plot for each modeled protein showed that over 92% of the residues were located in the most favourable region, with an average of 5.4% of the residues located in the allowed region while less than 0.4% were in the disallowed region (Supplementary Figure S3). Based on the analysis of 118 structures with a minimum of 2.0 Å and a maximum R-factor value of 20%, it is expected that a good quality model will have more than 90% of its resides in the most favored region.