Chain(s) Position Wild-type residues Mutant residues ΔΔGAffinity (Kcal/mol) Effect
H 219 LEU ALA -1.387 Decreasing affinity
H 225 VAL ALA -0.22 Decreasing affinity
H 233 LEU ALA -0.427 Decreasing affinity
H 234 LYS ALA -2.149 Decreasing affinity
H 238 LEU ALA -1.091 Decreasing affinity
M 94 LEU ALA -1.615 Decreasing affinity
M 163 LEU GLU -1.19 Decreasing affinity