Figure 5. EFEMP1 expression in COS7 cells.
A. Western blot of wild-type (WT) and mutant EFEMP1 proteins. Transfected COS7 cell-lysates showing expression of WT and mutant EFEMP1. Control group consist of cell-lysates from non-transfected COS7 cells. B. Ratio of intracellular EFEMP1 protein to total protein for wild type and each variant. Relative EFEMP1 abundance was calculated as the ratio between the EFEMP1 signal and the corresponding total protein signal in each sample measured using Revert 700 total protein stain. Three biological repeats were done for each EFEMP1 condition. Differences between mutant EFEMP1 and wildtype EFEMP1 were tested using Student’s t-test with Bonferroni correction. Error bars indicate the standard error.  *p<0.05, **p<0.001