De Novo assembly of a draft genome for the black-faced
spoonbill
We sequenced and conducted a de novo genome assembly to generate
a reference genome from a rescued male black-faced spoonbill. The
assembled genome is 1.19 Gb in length (genomic coverage = 42.5×) and
contains 2,243 scaffolds (870 scaffolds > 10 Kb, total
1.186 Gb; scaffold N50 of 4.2 Mb; 34,176 contigs; contigs N50 of 71.0
Kb; Table S1). This draft genome includes a total of 78.2 Mb (6.6% of
total genome) of repetitive sequences (Table S2). We found 25,315
predicted genes, 11,733 of which could be functionally annotated. In
total, we obtained 399.6 Mb of genic regions (33.6% of the total
genome) consisting of 365.2 Mb of introns and 34.4 Mb of coding regions.
The completeness of our draft genome is high (Table S3): we identified
93.0% (4,569) of the Benchmarking Universal Single-Copy Orthologs
v3.0(Waterhouse et al., 2018). Therefore, the completeness of our draft
genome is comparable to other published avian genomes, such as the draft
genome of horn lark (Mason, Pulgarin, Cadena, & Lovette, 2020).