Antibodies
The following antibodies were used in combination with EU-tagging or HPG
protein labeling: rat anti-Elav (Developmental Studies Hybridoma Bank
(DSHB)) at 1:10, mouse anti-Pros (DSHB) at 1:20, guinea pig anti-Miranda
(gift of C.Q. Doe) at 1:400, guinea pig anti-Udd (gift of M. Buszczak)
at 1:1000, mouse anti-PCNA (Santa Cruz Biotechnology) at 1:500, and
rabbit anti-phosphorylated histone H3 (Millipore Sigma) at 1:1000.
Alexa-fluor conjugated secondary antibodies (ThermoFisher) were used.