Drosophila genetics
The following lines were obtained from the Bloomington DrosophilaStock Center: Oregon-R-P2 (wildtype) (stock # 2376), insc-Gal4(stock # 8751), nSyb-Gal4 (stock #51635), wor-Gal4 ;Dr / TM3, Ubx-lacZ (stock #56553), and UAS-myr::tdTomato(stock #3222). For EC-tagging, Gal4 lines were crossed withUAS-CD:UPRT on the 3rd chromosome (stock # 77120). The UAS-Polr1B{RNAi} flies, constructed using the VALIUM20 vector [36], were a gift from Michael Buszczak.