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Figure 1 . TrxR activities in the supernatant of CCF, the
low-pressure fraction (< 0.8 bar), the high-pressure fraction
(> 0.8 bar) and the flush of the depth filtration of the
molecule 1 cell culture
Figure 2 . TrxR activities in the low-pressure fraction
(< 0.8 bar), the high-pressure fraction (> 0.8
bar) the flush of the depth filtration, and the cell lysate of the
molecule 2 cell culture
Figure 3 . NADPH concentration in the low-pressure fraction
(< 0.8 bar), the high-pressure fraction (> 0.8
bar), the flush of the depth filtration, and the cell lysate of the
molecule 2 cell culture
Figure 4 . The non-reduced SDS-PAGE of molecule2 incubated in
cell lysates with different NADPH concentrations. Lane: 1. Marker; 2.
Reduction by DTT; 3. NADPH >1.5 μM cell lysate; 4. NADPH
1.0 μM cell lysate; 5. NADPH 0.5 μM cell lysate. Reduction by DTT was
used as a positive control.
Figure 5 . NADPH concentration in the cell lysates and the
supernatants of CCF of five recombinant molecules
Figure 6 : NADPH concentration in cell lysates of three
recombinant molecules on different culture days.
Figure 7 . NADPH concentration in the cell lysate of the seed,
the cell lysate at harvest, and the supernatant of the CCF.